Inhibitor analysis of synapsis by resolvase
نویسندگان
چکیده
منابع مشابه
Synapsis and catalysis by activated Tn3 resolvase mutants
The serine recombinase Tn3 resolvase catalyses recombination between two 114 bp res sites, each of which contains binding sites for three resolvase dimers. We have analysed the in vitro properties of resolvase variants with 'activating' mutations, which can catalyse recombination at binding site I of res when the rest of res is absent. Site I x site I recombination promoted by these variants ca...
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The transposon Tn3 encodes a protein known as resolvase. During transposition, resolvase catalyses a site-specific recombination between two directly repeated copies of the transposon DNA [I]. Resolvase acts by binding to 120 bp sites on the DNA, known as res sites, each of which contains three sub-sites called I, I1 and 111 [2]. During recombination, three resolvase dimers bind co-operatively ...
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linked and identically oriented res sites (Reed, 1981). gd resolvase catalyzes recombination within a comRecombination involves the binding of resolvase diplex nucleoprotein structure containing at least 12 remers to the two res sites, their synapsis, double strand solvase subunits bound to two 114 bp res sites. The cleavage at the crossover points, and exchange and 2,39 interaction between res...
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15 صفحه اولTransposon targeting determined by resolvase.
The Mu-related transposon Tn5090, also called Tn402, was observed to be highly selective for targets clustered in or close to recombination sites of serine-type recombinases in plasmids R388 and RP1. Transposition to the par area of RP1 responded strongly to a deletion in the gene of resolvase ParA. A search in sequence databanks revealed further insertions of Tn5090/Tn402 close to different ge...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1989
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)84791-9